High-Performance Liquid Chromatography (HPLC) with UV / RI / FLD / DAD Detectors
At SIMA LABS, our advanced High-Performance Liquid Chromatography (HPLC) systems are equipped with multiple detector configurations including UV (Ultraviolet), RI (Refractive Index), FLD (Fluorescence Detector), and DAD (Diode Array Detector). This powerful combination enables high-sensitivity qualitative and quantitative analysis of a broad range of compounds, including active pharmaceutical ingredients (APIs), excipients, impurities, degradation products, preservatives, sugars, and fluorescent biomolecules. These detectors allow us to tailor our methods to specific compound characteristics, making HPLC a highly flexible and precise technique for analyzing complex liquid matrices across pharmaceutical, food, nutraceutical, cosmetic, and environmental applications.
Key Offerings:
- Multi-detector HPLC systems (UV, RI, FLD, DAD) for enhanced analytical versatility
- Accurate quantification of APIs, impurities, preservatives, and excipients
- Detection of non-UV absorbing compounds using RI and FLD detectors
- Stability, dissolution, and assay testing
- Method development, validation, and routine analysis
- Regulatory compliance with USP, ICH, FSSAI, and ISO standards
- High-throughput capabilities with rapid turnaround times
Pharmaceuticals
Assay of APIs, impurity profiling, dissolution studies, and stability testing following ICH and USP protocols.
Food and Beverages
Analysis of sugars, preservatives, colorants, and additives to ensure quality and safety.
Environmental Monitoring
Detection of organic pollutants, phenolic compounds, and contaminants in water and soil samples.
Nutraceuticals
Quantification of vitamins, amino acids, herbal extracts, and plant bioactives.
Cosmetics and Personal Care
Identification and quantification of active ingredients, preservatives, and degradation products.
Biotechnology
Characterization and quantification of peptides, proteins, and biotherapeutics in research and production environments.
Applications of HPLC
High-Performance Liquid Chromatography (HPLC) equipped with UV, RI, FLD, and DAD detectors is a high-resolution analytical technique widely utilized across pharmaceutical, nutraceutical, food, environmental, cosmetic, and biotechnological sectors. It enables accurate separation, identification, and quantification of a wide range of compounds—ensuring compliance, safety, and quality in complex formulations and sample matrices.
Each detector offers specific strengths:
UV is ideal for compounds with chromophores.
RI detects substances without UV absorption like sugars and alcohols.
FLD provides ultra-sensitive detection for naturally fluorescent compounds or fluorescent-labeled analytes.
DAD offers full spectral data, allowing for peak purity assessment and compound identification.
We can analyze a wide range of compounds including APIs, degradation products, preservatives, amino acids, plant extracts, colorants, and more.
Yes. All our methods are validated and compliant with relevant standards such as USP, ICH, FSSAI, and ISO guidelines.
Absolutely. SIMA Labs offers complete method development, optimization, validation, and routine testing tailored to specific product or regulatory requirements.
We routinely analyze pharmaceutical formulations, herbal extracts, food products, cosmetic samples, and environmental matrices.
Frequently Asked Questions
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